Infection of Infectious Spleen and Kidney Necrosis Virus and Viral Nervouse Necrosis in Seawater Fish Identifed Using Multiplex Polymerase Chain Reaction

نویسندگان

چکیده

Indonesia become a maritime country with large of ocean area and beach line marine species diversity reaches 37% the world’s fish. This makes have aquaculture potential that supported by an appropriate climate. However, challenge viral diseases caused Infectious Spleen Kidney Necrosis Virus (ISKNV) Viral Nervous (VNN) would hamper causing huge economic losses. Therefore, it is necessary to carry out early detection methods are efficient, fast, precise accurate identifying these disease. Here we developed multiplex-PCR (mPCR) method can detect simultaneously ISKNV VNN. The results this showed high sensitivity specificity using MCP CP gene target primers VNN, respectively. From total 353 samples seawater fish examined mPCR, positive following single infection VNN were 14 2, We also found 18 grouper was co-infection viruses. results, shows mPCR has efficiency, faster accuracy. founding possible be applied in laboratory testing or system field for

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ژورنال

عنوان ژورنال: Jurnal Sain Veteriner

سال: 2022

ISSN: ['2443-1583', '2407-3733']

DOI: https://doi.org/10.22146/jsv.73599